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Teach away from
bicinchoninic acid assay

Glossary entry

inglés term or phrase:

overconfluent

español translation:

sobreconfluente(s)

Added to glossary by RNAtranslator
May 24, 2008 00:10
16 yrs ago
2 viewers *
inglés term

overconfluent

inglés al español Medicina Biología (biotecnología/química, microbiología)
Senescence-associated B-Galactosidase Activity. A cellular senescence marker assay was performed as described by XXX. Briefly, cells were fixed with 2% formaldehyde/0.2% glutaraldehyde in PBS and then exposed to 1 mg/ml X-Gal in an iron buffer at pH 6. Dark blue precipitate forms in older cells proportionally to approaching senescence. Replicate wells were also stained at pH 7 as a negative control to show specificity of activity, and care was taken to avoid artifacts caused by overconfluent or quiescent cultures. Positively staining cells were expressed as percentage of total cells in independent wells.

Gracias de antemano
Proposed translations (español)
4 +3 sobreconfluentes
4 superconfluentes
Change log

May 24, 2008 00:50: RNAtranslator changed "Field" from "Técnico/Ingeniería" to "Medicina" , "Field (specific)" from "Medicina (general)" to "Biología (biotecnología/química, microbiología)"

Jun 7, 2008 04:04: RNAtranslator Created KOG entry

Proposed translations

+3
38 minutos
Selected

sobreconfluentes

Se refiere a que, una vez alcanzado el estado de confluencia, las células permanecen en cultivo, cuando en el procedimiento normal de cultivo de células adherentes en monocapa las células se despegan, de disgregan y se vuelven a sembrar una vez alcanzada la confluencia para evitar que el cultivo envejezca.

"se emplearon relaciones mayores de células efectoras/diana (E/D), pero se abandonaron porque las células Jurkat están sobreconfluentes a relaciones de E/D>1"
http://scholar.google.es/scholar?hl=es&lr=&ie=ISO-8859-1&q=s...
Peer comment(s):
agree almaruizpayan : almaruizpayan
42 minutos
Gracias almaruizpayan
agree Conchi Otaola : Sí, de acuerdo
7 horas
Gracias Conchi
agree Egmont
10 horas
Gracias AVRVM
Something went wrong...
4 KudoZ points awarded for this answer. Comment: "Selected automatically based on peer agreement."
7 horas

superconfluentes

hay tan pocos ejemplos como de "sobreconfluentes", pero a mí me suena mejor:

Resumenes 2004Mediante inmunocitoquí-mica, se vio que p8 presenta una localización nuclear en células subconfluentes, y citoplasmá-tica en células superconfluentes. ...
www.qb.fcen.uba.ar/resumenes2004.htm
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