über 0,2 µm sterilfiltrieren
English translation: filter (the solution) through a 0.2 µm filter under sterile conditions
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| 11:26 Apr 28, 2007 |
| German to English translations [PRO] Science - Chemistry; Chem Sci/Eng / Raw Material SOP | |||||||
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|  Selected response from: Inge Grueneberg Germany Local time: 20:35 | ||||||
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| 4 +5 | sterile filter the solution over a 0.2 µm filter |
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Answers
19 mins confidence: 
peer agreement (net): +5
peer agreement (net): +5sterile filter the solution over a 0.2 µm filter Explanation: meant is the process of filtering through a pore size of 0.2 µm, which largely eliminates bacteria, since they are usually larger in size. The filtering assembly is variable. For small volumes you may use a syringe onto which one can fix a disposable syringe filter. Or you have a complete presteriled filter device with funnel, filter (0.2 µm) and container. Anyway you always sterile filter your solution over a 0.2 µm pore size filter, actually one may leave out the last part and just say sterile filter the solution, however since it is an SOP... Actually the instruction is incorrect. If you sterile filter first and then transfer the soultion to another vessel it becomes unsterile again. One should sterile filter the solution into a sterile PP Flask... If that aint enough! |
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