ADN templado

English translation: template DNA

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
Spanish term or phrase:ADN templado
English translation:template DNA
Entered by: SandraV

22:55 Dec 7, 2005
Spanish to English translations [PRO]
Science - Biology (-tech,-chem,micro-) / Biotechnology studies
Spanish term or phrase: ADN templado
¿¿En este caso se refiere a la temperatura del DNA, o a algún procedimiento especial??

La mezcla de reacción usada para la PCR contenía: 100 ng de ******ADN templado****** de P. capsici, 0.2 mM de cada iniciador, 2.5 mM de cada dNTP, 15 mM MgCl2, 100 mM Tris-HCl (pH 8), 500 mM KCl, 3 unidades de Taq polimerasa (Boehringer, Mannheim, Indianapolis, 1N), en un volumen final de 50 l (Medina, 2002).
SandraV
Mexico
Local time: 07:32
template DNA
Explanation:
Could it be refering to a template strand of DNA to be used in a PCR (polimerase chain reaction)?
I used to work in a lab doing PCRs only and we used DNA templates to replicate it.


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Note added at 59 mins (2005-12-07 23:54:55 GMT)
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Protocol for PCR using Taq DNA PolymeraseA control reaction, omiting template DNA, should always be performed, ... The number of PCR cycles depends on the amount of ***template DNA*** in the reaction mix ...
www.fermentas.com/techinfo/pcr/dnaamplprotocol.htm

Revista chilena de infectolog - <I>Biolog molecular en ... - [ Translate this page ]
Cada hebra de ***ADN (templado)*** sirve como molde para la amplificación y sólo ...
El ADN doble hebra formado (templado: partidor) definirá el sitio de acción ...
http://www.scielo.cl/scielo.php?pid=S0716-10182002000100003&...

Preparation of ***Template for DNA Sequencing***The quality of the DNA template is one of the most critical factors in ... If you have enough template DNA, always determine its concentration by UV ...
seqcore.brcf.med.umich.edu/doc/dnaseq/dnaprep.html
Selected response from:

PaulinaRich
United States
Local time: 07:32
Grading comment
¡Muchas gracias, Paulina y a todos por su ayuda!
4 KudoZ points were awarded for this answer



Summary of answers provided
5 +3template DNA
PaulinaRich
5templateD DNA
celiacp
3room-temperature DNA
Robert Forstag


  

Answers


2 mins   confidence: Answerer confidence 3/5Answerer confidence 3/5
room-temperature DNA


Explanation:
The term refers to the temperature of the DNA being used.

Suerte.


    Reference: http://www.chem.umass.edu/~cmartin/Pubs/PDF/McIntosh_AuNanoD...
    www.genvault.com/news/isber.htm -
Robert Forstag
United States
Local time: 09:32
Native speaker of: English
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55 mins   confidence: Answerer confidence 5/5 peer agreement (net): +3
template DNA


Explanation:
Could it be refering to a template strand of DNA to be used in a PCR (polimerase chain reaction)?
I used to work in a lab doing PCRs only and we used DNA templates to replicate it.


--------------------------------------------------
Note added at 59 mins (2005-12-07 23:54:55 GMT)
--------------------------------------------------

Protocol for PCR using Taq DNA PolymeraseA control reaction, omiting template DNA, should always be performed, ... The number of PCR cycles depends on the amount of ***template DNA*** in the reaction mix ...
www.fermentas.com/techinfo/pcr/dnaamplprotocol.htm

Revista chilena de infectolog - <I>Biolog molecular en ... - [ Translate this page ]
Cada hebra de ***ADN (templado)*** sirve como molde para la amplificación y sólo ...
El ADN doble hebra formado (templado: partidor) definirá el sitio de acción ...
http://www.scielo.cl/scielo.php?pid=S0716-10182002000100003&...

Preparation of ***Template for DNA Sequencing***The quality of the DNA template is one of the most critical factors in ... If you have enough template DNA, always determine its concentration by UV ...
seqcore.brcf.med.umich.edu/doc/dnaseq/dnaprep.html


PaulinaRich
United States
Local time: 07:32
Specializes in field
Native speaker of: Native in SpanishSpanish
PRO pts in category: 4
Grading comment
¡Muchas gracias, Paulina y a todos por su ayuda!

Peer comments on this answer (and responses from the answerer)
agree  Georges Tocco: definitely
1 min
  -> thanks Georges

agree  Sonia Iujvidin
14 hrs
  -> thanks soniadin

agree  Andrés Martínez: Así es, un amigo que hace PCR en un laboratorio me confirma que debe tratarse de una mala traducción de "template" (molde, plantilla). La temperatura adecuada la aplica el termociclador.
2 days 9 hrs
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11 hrs   confidence: Answerer confidence 5/5
templateD DNA


Explanation:
MCDB 2150 -- Lecture 3 - [ Traduzca esta página ]
Templated DNA synthesis: After the RNA primer has reached an adequate length,
DNA polymerase III begins synthesis of DNA, which proceeds to completion in ...
www.colorado.edu/MCDB/MCDB2150Fall/notes00/L0003.html - 22k - En caché - Páginas similares

Ellenberger Lab Research - [ Traduzca esta página ]
We have a long-standing interest in the mechanism of templated DNA synthesis by
DNA polymerases. We are investigating bacteriophage T7 DNA replication ...
biochem.wustl.edu/~tome/research.htm - 15k - 6 Dic 2005 - En caché - Páginas similares

Ahí encontrarás la explicación de lo que es el ADN templado. Suerte en tu traducción!

celiacp
Spain
Local time: 15:32
Native speaker of: Spanish
PRO pts in category: 16
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