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  3. Biology (-tech,-chem,micro-)

mispriming

English translation: \"mispriming\"/\"misprajming\"/pogrešno započinjanje

GLOSSARY ENTRY (DERIVED FROM QUESTION BELOW)
Serbian term or phrase:mispriming
English translation:\"mispriming\"/\"misprajming\"/pogrešno započinjanje
Entered by: Radica

20:36 Jun 11, 2013
Serbian to English translations [PRO]
Medical - Biology (-tech,-chem,micro-) / Mikrobiologija
Serbian term or phrase: mispriming
U pitanju je uputstvo za koriscenje kompleta za brzu prenatalnu dijagnostiku trizomije i aneuploidije polnih hromozoma

The enzyme is completely inactive at room temperature. This prevents MISPRIMING during Polymerase Chain Reaction set up. Activation is obtained during the 15 min. at 95ºC step before PCR cycling. This simplifies PCR set up and handling that can easily be done at room temperature.
Radica
Local time: 03:50
"mispriming"/"misprajming"/pogrešno započinjanje
Explanation:
Izvinjavam se za više odgovora, ali zapravo su svi odgovarajući. Mispriming dolazi od riječi "primer", (na srpskom često prevedeno "prajmer"). Koliko znam, ne postoji službeni prijevod ovog termina u znanstvenoj literaturi. Zato predlažem "mispriming" pod navodnicima ili po uzoru na "prajmer", "misprajming". Radi se o pogrešnom započinjanju kod lančane reakcije polimeraze.

Ako netko ima bolju informaciju o službenom prijevodu, neka javi, jer i mene zanima.

Mispriming is the result of a primer binding to an unintended template resulting in amplification. The amplicon can sometimes be the same size as the intended product, but is usually a different size when viewed following agarose gel electrophoresis. Mispriming occurs because of poorly optimised conditions or because we haven't checked whether our sequence will inadvertently bind to an entirely different target entity e.g. a region of the human genome instead of the intended virus genome.
Selected response from:

Nikola Bijelić, MD, PhD
Croatia
Local time: 03:50
Grading comment
hvala!
4 KudoZ points were awarded for this answer



Summary of answers provided
5 -1lažan rezultat
Milena Nikolić
4"mispriming"/"misprajming"/pogrešno započinjanje
Nikola Bijelić, MD, PhD


  

Answers


13 hrs   confidence: Answerer confidence 5/5 peer agreement (net): -1
lažan rezultat


Explanation:
prim

Precise or proper to the point of affectation; excessively decorous.

ovdje je u pitanju čitanje rezultata

Milena Nikolić
Local time: 03:50
Native speaker of: Native in MontenegrinMontenegrin, Native in SerbianSerbian

Peer comments on this answer (and responses from the answerer)
disagree  Nikola Bijelić, MD, PhD: Nažalost, to nije taj termin. Vidite: http://www.uq.edu.au/vdu/PDU_PCRPrimers.htm Pozdrav!
2 hrs
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15 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
"mispriming"/"misprajming"/pogrešno započinjanje


Explanation:
Izvinjavam se za više odgovora, ali zapravo su svi odgovarajući. Mispriming dolazi od riječi "primer", (na srpskom često prevedeno "prajmer"). Koliko znam, ne postoji službeni prijevod ovog termina u znanstvenoj literaturi. Zato predlažem "mispriming" pod navodnicima ili po uzoru na "prajmer", "misprajming". Radi se o pogrešnom započinjanju kod lančane reakcije polimeraze.

Ako netko ima bolju informaciju o službenom prijevodu, neka javi, jer i mene zanima.

Mispriming is the result of a primer binding to an unintended template resulting in amplification. The amplicon can sometimes be the same size as the intended product, but is usually a different size when viewed following agarose gel electrophoresis. Mispriming occurs because of poorly optimised conditions or because we haven't checked whether our sequence will inadvertently bind to an entirely different target entity e.g. a region of the human genome instead of the intended virus genome.

Example sentence(s):
  • Sinteza obilježenog DNA markera provodi se metodom nasumičnog započinjanja (engl.random priming)...

    Reference: http://www.uq.edu.au/vdu/PDU_PCRPrimers.htm
    Reference: http://en.wiktionary.org/wiki/mispriming
Nikola Bijelić, MD, PhD
Croatia
Local time: 03:50
Specializes in field
Native speaker of: Native in CroatianCroatian
PRO pts in category: 8
Grading comment
hvala!
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