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Medical translator and former scientist specialising in academic and scientific translation in the fields of medicine, life sciences and regulatory affairs
Account type
Freelance translator and/or interpreter, Verified member
German to English: PAR1 Rezeptoren vermitteln die neuronale Aktivierung durch Biopsieüberstände der Kolonschleimhaut von Patienten mit Reizdarmsyndrom (IBS) aber nicht von Patienten mit Colitis Ulcerosa (CU) General field: Medical Detailed field: Science (general)
Source text - German PAR1 Rezeptoren vermitteln die neuronale Aktivierung durch Biopsieüberstände der Kolonschleimhaut von Patienten mit Reizdarmsyndrom (IBS) aber nicht von Patienten mit Colitis Ulcerosa (CU)
Hintergrund: Mediatoren der Kolonscheimhaut von IBS Patienten aktivieren Neurone des humanen submucösen Plexus. Diese Aktivierung wird überwiegend durch Histamin, Serotonin und Proteasen vermittelt. Für Histamin und Serotonin konnten die beteiligten Rezeptoren identifiziert werden, für die Proteasen bisher nicht. Wir konnten kürzlich zeigen, dass neuronal Proteaseeffekte am menschlichen Darm überwiegend durch den Protease-Aktivierten Rezeptor (PAR) 1 vermittelt werden.
Methode: Daher untersuchten wir in der vorliegenden Arbeit die Wirkung des PAR1 Rezeptorantagonisten SCH79797 (10µM) auf die neuronale Aktivierung durch Schleimhautbiopsieüberstände von 10 Patienten mit IBS (9 IBS-D und 1 IBS-C). Die neuronale Aktivität wurde mittels eines Neuroimaging-Verfahrens unter Verwendung eines spannungssensitiven Farbstoffs (Di-8-ANEPPS) an 233 humanen submucösen Neuronen gemessen. In derselben Weise wurden die Effekte von Kolonschleimhautüberständen von 9 Patienten mit CU in Remission untersucht.
Ergebnisse: Alle IBS Überstände aktivierten die humanen submukösen Neurone (mediane Aktionspotentialfrequenz 2,9Hz, IQR 1,2–5,3Hz; n=142 Neurone). Der PAR1 Rezeptor-antagonist SCH79797 hemmte diese Aktivierung signifikant für jede Probe (Median 0Hz (IQR 0–1,6Hz)). Dies steht in deutlichen Gegensatz zu den Befunden für die entsprechenden Überstände der CU Patienten. Hier konnte die starke nervenaktivierende Wirkung der Überstände nicht durch den PAR1 Rezeptorantagonist gehemmt werden (mediane Aktionspotentialfrequenz 2,4 (IQR 1,7–4,8) vs. 2,4 (IQR 1,3–4,2) Hz, n=91 Neurone).
Schlussfolgerung: Bei IBS scheinen neuronale PAR1 vermittelte Effekte eine wichtige Rolle bei der Aktivierung enterischer Neurone durch mucosale Faktoren zu spielen. Die Ergebnisse sprechen dafür, dass Änderungen im Signalling zwischen der Darmschleimhaut und submukösen Neuronen auch bei CU in Remission existieren. Jedoch ist das Wirkprofil der Mediatoren ein anderes als bei IBS. Für die Behandlung des IBS könnte PAR1 ein attraktives Target darstellen.
(Source: Z Gastroenterol 2012; 50 - K245)
Translation - English PAR1 receptors mediate the neural activation by supernatants in colonic biopsy specimens from patients with Irritable Bowel Syndrome (IBS) but not from patients with Colitis Ulcerosa (CU)
Background: Mediators released by the colonic mucosa of IBS patients activate neurons of the human submucosal plexus. This activation is predominantly mediated by histamine, serotonin and proteases. The receptors involved in the signalling of histamine and serotonin have been identified, but those involved in the effect of proteases have not. We have recently demonstrated that protease-activated receptor (PAR) 1 mediates the neuronal effects of proteases in the human intestine.
Methods: In the present study, we have therefore investigated the effect of the PAR1 receptor antagonist SCH79797(10µM) on the neuronal activation by supernatants of mucosal biopsy specimens from 10 IBS patients (9 IBS-D and 1 IBS-C). The neuronal activity was measured in 233 human submucosal neurons by neuroimaging using a voltage-sensitive dye (Di-8-ANEPPS). Likewise, the effect of colonic mucosal biopsy supernatants from 9 CU patients in remission was measured.
Results: All IBS supernatants activated human submucosal neurons (median frequency of action potential discharge: 2.9Hz, IQR 1.2–5.3Hz; n=142 neurons). The PAR1 receptor antagonist SCH79797 inhibited this activation significantly in each sample (median: 0Hz (IQR 0–1.6Hz)). This is in marked contrast to the results obtained with the respective supernatants from CU patients. Here the potent neuronal activation by the supernatants could not be inhibited by the PAR1 receptor antagonist (median frequency of action potential discharge: 2.4 (IQR 1.7–4.8) vs. 2.4 (IQR 1.3–4.2) Hz, n=91 neurons).
Conclusions: In IBS, neuronal PAR1-mediated effects appear to play an important role in the activation of enteric neurons by mucosal factors. The results indicate the presence of alternations in the signalling between the intestinal mucosa and submucosal neurons also during remission of CU. However, the mode of action of the mediators differs from that in IBS. PAR1 could present an attractive target for the treatment of IBS.
(Source: Z Gastroenterol 2012; 50 - K245)
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Translation education
PhD - Utrecht University, the Netherlands
Experience
Years of experience: 13. Registered at ProZ.com: Sep 2013. Became a member: Nov 2014.
Credentials
German to English (PhD, Utrecht University ) Dutch to English (PhD, Utrecht University)
Freelance medical translator. German and Dutch to English. Offering expertise in Medicine and Life Sciences in combination with language skills and cultural knowledge acquired by international upbringing.
Profile:
● PhD in Physiology and Immunology
● Native proficiency in English and Dutch acquired growing up in a bilingual household in the Netherlands.
● Near-native proficiency in German acquired growing up in Germany
● Over eight years of work experience in biomedical research in academia
● Experience in writing, editing, reviewing and publishing of medical research publications
● Author of several research publications in major international journals in the field of surgery, gastroenterology, parasitology, and physiology
Work Experience:
● Freelance translator since 2011
● Guest Professor, Pompeu Fabra University, Mataro, Spain
● Dissertation director, Autonomous University of Barcelona, Barcelona, Spain (link)
● Postdoctoral researcher, Autonomous University of Barcelona, Barcelona, Spain
● PhD candidate, Institute for Risk Assessment Sciences (IRAS), Utrecht University, Utrecht, the Netherlands
● Quality Assurance Coordinator, Department of pharmaceutical affairs, Sandoz/Novartis group, Almere, the Netherlands
Education:
● Ph.D: Physiology and Immunology, Utrecht University, the Netherlands
● M.Sc: Medical Biology, Radboud University, the Netherlands
● B.Sc: Medical Biology, Radboud University, the Netherlands
Keywords: German to English medical translation, translation of medical research articles, translation of scientific research articles, medical translator German to English, medical translator Dutch to English, specialist medical and pharmaceutical translations, medical translation revision, chemical translations, translation of life science texts, translation of medical documents. See more.German to English medical translation, translation of medical research articles, translation of scientific research articles, medical translator German to English, medical translator Dutch to English, specialist medical and pharmaceutical translations, medical translation revision, chemical translations, translation of life science texts, translation of medical documents, medical technology translations. See less.
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