-------------------------------------------------- Note added at 1 hr 3 mins (2005-07-15 11:43:37 GMT) --------------------------------------------------
General Filter Products - [ Traduci questa pagina ]
... sedimentation, coagulation, flocculation, separation, oxidation, thickening,
pressure and gravity filtration, packaged treatment equipment. ... www.usfilter.com/water/Business+Centers/ General_Filter_Products/ - 38k - Copia cache - Pagine simili
-------------------------------------------------- Note added at 1 hr 6 mins (2005-07-15 11:46:06 GMT) --------------------------------------------------
International Pbi Spa
Metodi di filtrazione - La filtrazione può avvenire per gravità, vuoto o pressione.
P Porta filtro - Dispositivo per la filtrazione continua e discontinua ... www.internationalpbi.it/default. php?t=site&pgid=806&lang=IT - 37k - Copia cache - Pagine simili
-------------------------------------------------- Note added at 1 hr 41 mins (2005-07-15 12:21:43 GMT) --------------------------------------------------
The liposome used in the present invention may be prepared by conventional methods. For example, a mixture of the above-mentioned lipids, from which the solvents have been removed, is emulsified by the use of a homogenizer, lyophilized, and melted to obtain multilamera liposome. Adjustment of particle size of the resultant liposomes may be conducted by ultrasonication, high-speed homogenization, or pressure filtration through a membrane having uniform pore size (Hope M. J. et al., Biochimica et Biophysica Acta 812 55, 1985). Preferable particle size of the liposomes are between 30 nm and 200 nm. http://www.pharmcast.com/Patents100/Yr2004/Sept2004/090704/6...
BK and Ricin Antibodies
Monoclonal antibodies against BK were obtained using a hybridoma cell line (SBK-1) kindly provided by M. Web (Sandoz, Sandwich, UK). Monoclonal antibodies were purified from ascites fluid of BALB/B mice treated with the hybridoma. Briefly, after clarification by centrifugation at 9000 rpm for 10 minutes, the ascites fluid was diluted 1:1 with 20 mmol/L phosphate buffer, pH 7.0. The IgG fraction was obtained by adsorption to and elution from a Fast Flow Protein G column (Pharmacia). The IgG fraction was equilibrated against PBS, pH 7.4, and concentrated by pressure-filtration using an Amicon Cell Concentrator (Amicon). Final protein concentration was 19 mg/mL. In a kinin radioimmunoassay15 a dilution of 1 000 000 of the antibody bound approximately 90% of 125I-Ttr8-bradykinin. The purified IgG fraction monoclonal antibodies against ricin (a plant structural component) were used as controls to account for possible nonspecific actions of the monoclonal antibodies. Ricin monoclonal antibodies were produced as previously described.16 http://hyper.ahajournals.org/cgi/content/full/30/5/1105
Example 7: Ozone Treatment of an Organic Solution of PHA
[0043] Cells of P. putida fermented, as described above, on octanoic acid were collected by centrifugation and freeze-dried to give a solid mass. The mass (440 g) was pulverized in a mill and placed in the thimble of a Soxhlet extractor. The dried cells were extracted in two batches for a total of 16 hr with n-hexane (2 L), yielding an amber extract (A.sub.273=0.457, 1:9 dilution in hexane) which contained 26% (wt/vol) solids. The extract was clarified by pressure filtration through a 2 .mu.m glass microfiber filter. The extract (26% wt/vol solids) then was sparged with an ozone-containing oxygen gas stream (8 standard cubic feet per hour, ca. 2% ozone) for 15 min at room temperature with agitation. The resulting product was a clear, virtually colorless liquid (A.sub.273=0.152, 1:9 dilution in hexane). A portion of ozonated extract (0.6 L) then was passed through a column of silica gel (ca. 60 g), and the colorless eluate (26% wt/vol solids) was collected (A.sub.2730.063, 1:9 dilution in hexane).
Example 8: Purification of PHAs Dissolved in Dichloromethane
[0044] Solutions of poly-3-hydroxybutyrate (PHB) (Fluka) and poly-3-hydroxybutyrate-co-14%-3-hydroxyvalerate (PHBV) (Aldrich) were prepared at 5% (wt/vol) in dichloromethane. The solutions were clarified by pressure filtration through a 2.6-.mu.m glass microfiber filter. Each solution (95 mL) was then sparged with an ozone-containing oxygen gas stream (8 standard cubic feet per hour, ca. 2% ozone) for 6 min at room temperature with agitation. The products were clear, virtually colorless solutions. The PHB solution after ozonation showed an A.sub.273=0.714 (1:9 dilution in dichloromethane) compared to A.sub.273=0.904 (1:9 dilution in dichloromethane) prior to ozonation. Similarly, the PHBV solution after ozonation showed an A.sub.273=0.599 (1:9 dilution in dichloromethane) compared to A.sub.273=1.029 (1:9 dilution in dichloromethane) prior to ozonation.
56 minutos Nivel de confianza: Coincidencias de otros usuarios (netas) +1
yes
Explicación: I'd say pressure filtration
-------------------------------------------------- Note added at 1 hr 3 mins (2005-07-15 11:43:37 GMT) --------------------------------------------------
General Filter Products - [ Traduci questa pagina ]
... sedimentation, coagulation, flocculation, separation, oxidation, thickening,
pressure and gravity filtration, packaged treatment equipment. ... www.usfilter.com/water/Business+Centers/ General_Filter_Products/ - 38k - Copia cache - Pagine simili
-------------------------------------------------- Note added at 1 hr 6 mins (2005-07-15 11:46:06 GMT) --------------------------------------------------
International Pbi Spa
Metodi di filtrazione - La filtrazione può avvenire per gravità, vuoto o pressione.
P Porta filtro - Dispositivo per la filtrazione continua e discontinua ... www.internationalpbi.it/default. php?t=site&pgid=806&lang=IT - 37k - Copia cache - Pagine simili
-------------------------------------------------- Note added at 1 hr 41 mins (2005-07-15 12:21:43 GMT) --------------------------------------------------
The liposome used in the present invention may be prepared by conventional methods. For example, a mixture of the above-mentioned lipids, from which the solvents have been removed, is emulsified by the use of a homogenizer, lyophilized, and melted to obtain multilamera liposome. Adjustment of particle size of the resultant liposomes may be conducted by ultrasonication, high-speed homogenization, or pressure filtration through a membrane having uniform pore size (Hope M. J. et al., Biochimica et Biophysica Acta 812 55, 1985). Preferable particle size of the liposomes are between 30 nm and 200 nm. http://www.pharmcast.com/Patents100/Yr2004/Sept2004/090704/6...
BK and Ricin Antibodies
Monoclonal antibodies against BK were obtained using a hybridoma cell line (SBK-1) kindly provided by M. Web (Sandoz, Sandwich, UK). Monoclonal antibodies were purified from ascites fluid of BALB/B mice treated with the hybridoma. Briefly, after clarification by centrifugation at 9000 rpm for 10 minutes, the ascites fluid was diluted 1:1 with 20 mmol/L phosphate buffer, pH 7.0. The IgG fraction was obtained by adsorption to and elution from a Fast Flow Protein G column (Pharmacia). The IgG fraction was equilibrated against PBS, pH 7.4, and concentrated by pressure-filtration using an Amicon Cell Concentrator (Amicon). Final protein concentration was 19 mg/mL. In a kinin radioimmunoassay15 a dilution of 1 000 000 of the antibody bound approximately 90% of 125I-Ttr8-bradykinin. The purified IgG fraction monoclonal antibodies against ricin (a plant structural component) were used as controls to account for possible nonspecific actions of the monoclonal antibodies. Ricin monoclonal antibodies were produced as previously described.16 http://hyper.ahajournals.org/cgi/content/full/30/5/1105
Example 7: Ozone Treatment of an Organic Solution of PHA
[0043] Cells of P. putida fermented, as described above, on octanoic acid were collected by centrifugation and freeze-dried to give a solid mass. The mass (440 g) was pulverized in a mill and placed in the thimble of a Soxhlet extractor. The dried cells were extracted in two batches for a total of 16 hr with n-hexane (2 L), yielding an amber extract (A.sub.273=0.457, 1:9 dilution in hexane) which contained 26% (wt/vol) solids. The extract was clarified by pressure filtration through a 2 .mu.m glass microfiber filter. The extract (26% wt/vol solids) then was sparged with an ozone-containing oxygen gas stream (8 standard cubic feet per hour, ca. 2% ozone) for 15 min at room temperature with agitation. The resulting product was a clear, virtually colorless liquid (A.sub.273=0.152, 1:9 dilution in hexane). A portion of ozonated extract (0.6 L) then was passed through a column of silica gel (ca. 60 g), and the colorless eluate (26% wt/vol solids) was collected (A.sub.2730.063, 1:9 dilution in hexane).
Example 8: Purification of PHAs Dissolved in Dichloromethane
[0044] Solutions of poly-3-hydroxybutyrate (PHB) (Fluka) and poly-3-hydroxybutyrate-co-14%-3-hydroxyvalerate (PHBV) (Aldrich) were prepared at 5% (wt/vol) in dichloromethane. The solutions were clarified by pressure filtration through a 2.6-.mu.m glass microfiber filter. Each solution (95 mL) was then sparged with an ozone-containing oxygen gas stream (8 standard cubic feet per hour, ca. 2% ozone) for 6 min at room temperature with agitation. The products were clear, virtually colorless solutions. The PHB solution after ozonation showed an A.sub.273=0.714 (1:9 dilution in dichloromethane) compared to A.sub.273=0.904 (1:9 dilution in dichloromethane) prior to ozonation. Similarly, the PHBV solution after ozonation showed an A.sub.273=0.599 (1:9 dilution in dichloromethane) compared to A.sub.273=1.029 (1:9 dilution in dichloromethane) prior to ozonation.
The translation workplace 
Coincidencias de otros usuarios (netas) +1